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W130i DLS (Dynamic Light Scattering)
System W130i ³ª³ëÆÄƼŬ ÀÔµµ ºÐ¼®±â

 

 

 

W130i Dynamic Light Scattering System ÀÇ ÁÖ¿äƯ¡
1. Á¤È®ÇÑ ÆÄƼŬ »çÀÌÁî ¿µ¿ª  –  0.25nm to 1000nm(diameter) ÃÖ°í°¨µµ µðÅØÅÍÀÎ APD »ç¿ë
2.  8X Faster BladeCell(ƯÇã)ÀÇ »ç¿ëÀ¸·Î »ç¿ëÀÌ ¸Å¿ìÆí¸®ÇÏ¸ç ±âÁ¸ÀÇ ¼¿·Î »ùÇÃÃøÁ¤Áغñ±îÁö
    ¾à 4ºÐÀÌ»ó °É·ÈÀ¸³ª BladeCellÀº ´Ü 30ÃÊ¿¡ ¿Ï·áÇÏ¿© ±âÁ¸ÀÇ Á¦Ç°º¸´Ù 8¹è »¡¸® ÃøÁ¤Áغñ¸¦ ÇÒ¼ö
    ÀÖ½À´Ï´Ù.

3. ÃÖ¼Ò 0.2ulÀÇ ±Ø¼Ò·®ÀÇ »ùÇþçÀ¸·ÎºÎÅÍ SEC¿ë FlowCell, 1.5mlÀÇ ´Ù¾çÇÑ »ùÇü¿À» °¡Áö°í À־ ´Ù¾çÇÑ
  »ùÇÃÀ» »ç¿ëÇϽǼö ÀÖ½À´Ï´Ù.
4. Æí¸®ÇÑ i-Size ¼ÒÇÁÆ®¿þ¾î – ´Ù¾çÇÑ ÃøÁ¤°á°ú¸¦ È®ÀÎ
5. ³ÐÀº ¿ÂµµÃøÁ¤¿µ¿ª 0° to 90°C
  - SEC (Chromatograph Online ¿¬°á)À¸·Î ±¸¼ºÇÏ¿© ½Ç½Ã°£À¸·Î ÀÔµµÃøÁ¤
  (LS –DetectorÀ¸·Î »ç¿ë)¹× »ùÇÃaggregration ½Ç½Ã°£È®ÀÎ.
6. Non-invasive – no column separation required.
7. ¸Å¿ìºü¸¥ ÃøÁ¤½Ã°£ - 1ºÐÀ̳» ÃøÁ¤¿Ï·á
8. ¿Â¶óÀÎ ½Ç½Ã°£ »ùÇÃÃøÁ¤°¡´É-
9. ISO compliant measurements – ISO13321, ISO22412

10. »ç¿ëÀÚ¿¡ Æí¸®ÇÑ i-Size Software
 

 

http://www.youtube.com/watch?v=PbFVxFMoNKk – °£´ÜÇÑ 4step ÃøÁ¤.
http://www.youtube.com/watch?v=0YWEvbRZJLs – ´Ù¾çÇÑ »ùÇü¿ ¼±ÅÃ,»ç¿ë.
http://www.youtube.com/watch?v=Xn8nOUfJqgI – 20nm + 100nm°­·ÂÇÑ ºÐº°·Â

 

 

    Protein purification and buffer formulation
     Aggregation
     Molecular weight
     Micelle formation
     Temperature denaturing
     Nano Particle(³ª³ëÀÔÀÚ ÃøÁ¤), Colloidals

 

 

W130i Specifications(Á¦Ç°»ç¾ç)

Measurement Principle

Dynamic Light Scattering
Measurement Range
(hydrodynamic diameter)
0.25 to 1000nm
Measurement Time (typical) 30 sec
Sample Liquid Volume
(min – max)
2 – 4500μL & online
Light Source 660nm; 30mW
Detector Type Avalanche Photo Diode
Temperature Control Range 0°C – 90°C
Temperature Control Accuracy ±0.2°C
Cuvette Type quartz Cell or BladeCell, FlowCell…
Computer Communication 1 x USB
Power Supply Temperature: 10°C to 40°C
  Humidity: 10% to 90% (without condensation)
Power Supply AC 100V ~ 240V, 50/60 Hz universal input
Size and Weight 130 (W) x 340 (D) x 210 (H), 6.5kg
Computer OS Microsoft Windows XP / 7 (32 or 64 bit)
Validation Standards ISO13321 : 1996
  ISO22412 : 2008
Accuracy ±2%
Polydispersity  Index <0.1 (certified standard)
 

0.1g/L of lysozyme protein in phosphate buffer
(2nm radius)

 

(much lower sample concentration required for
larger particles)

  192 Da (e.g. caffeine) ~ 25MDa
  2μL (partially filled 12μL quartz cuvette)
  0 – 90 deg C

Sample cuvette
types (typical)

5μL polycarbonate ‘BladeCell’ disposable (Avid Nano design)

  Generic 12.5mm square walled cuvettes with 4 clear windows
  8μL flow through cell (option)
  12μL quartz
  45μL quartz
  50μL polystyrene disposable (Eppendorf Uvette)
Measurements provided

Mean hydrodynamic size (nm)
(according to ISO13321)

  Polydispersity Index (according to ISO13321)
  Standard deviation (nm)
  Mean photon count rate
  Intensity Size Distribution
  Mass Size Distribution
  Peak distribution, SD, %mass analysis
 

Molecular Weight estimate
(for biological molecules)

 

Molecular Weight measurement by batch
static light scattering analysis

 

 

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